Dominant Negative α-Subunit of FTase Inhibits Effects of Insulin and IGF-I in MCF-7 Cells
作者： Curtis Scott Solomon , Marc Lee Goalstone
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摘要: We recently designed a dominant negative (DN) farnesyltransferase (FTase)/geranyl-gerahyltransferase I (GGTase I) α-subunit that when expressed in vascular smooth muscle cells decreased insulin-stimulated phosphorylation of FTase, FTase activity, amounts farnesylated p21Ras, DNA synthesis, and cell migration. Currently, we explored the inhibitory effects DN FTase/GGTase MCF-7 on IGF-1- synthesis proliferation. Expression completely blocked BrdU incorporation count. inhibited α-subunit, GGTase prenylation p21Ras RhoA. diminished ERK (extracellular signal-regulated kinase), but had no effect Akt. Taken together, these data suggest can assuage mitogenic IGF-1 insulin breast cancer cells.
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