Radiodetoxified Endotoxin—Induced Tolerance Alters Monocyte but Not Neutrophil CD11b and CD18 Expression in Response to Lipopolysaccharide

摘要: Objectives: To test the hypothesis that pretreatment with radiodetoxified endotoxin (RDE) may mitigate deleterious effects of subsequent infection, in part by modifying leukocyte adhesion receptor expression, and to investigate cellular mechanisms tolerance induced RDE. Design: assess effect RDE on mortality from bacterial peritonitis, rats were implanted an intraperitoneal, barium-fecal inoculum at intervals 0,1, 3, 5 days after injection. Experiments then conducted expression. Two groups mice received saline solution, one group, After 72 hours, group solution (saline/saline group), others, lipopolysaccharide (LPS) (saline/LPS RDE/LPS groups). Peripheral leukocytes obtained 1 hour injection analyzed for CD11b CD18 expression flow cytometry. Setting: Laboratory animal study. Results: Survival rates not improved pretreated 0 24 hours before (0% 7%, respectively). In 120 inoculum, 47% (P Conclusions: Radiodetoxified reduces peritonitis when given least prior a inoculum. Tolerance LPS challenge is associated abrogation reduced peripheral monocyte CD 18 observed native LPS-stimulated but changes polymorphonuclear 11b The mechanism RDE-induced hyporesponsiveness its possible protective uncertain requires further investigation. (Arch Surg. 1994;129:1153-1158)