The Co-Transcriptional Response of Intracellular Group B Streptococcus (GBS) and Monocytes

摘要: Group B Streptococcus (GBS) is a species of gram positive bacteria representing significant human pathogen; namely, as the most prolific cause neonatal disease and mortality globally, but also increasingly reported in adult (especially among elderly those with compromised immune systems). The first chapter this thesis reviews extensive literature covering GBS; focus on classification disease. Selected virulence factors are discussed. In 2, eight strains GBS were selected for whole genome sequencing by Third Generation, Pacific Biosystems (PacBio) technology. A protocol was optimised to provide sufficient, high quality genomic preparations from multiple – suitable PacBio Using sequenced strain an infection model 3 purpose providing RNA co-transcript analysis. U937 monocytes infected (strain 874391) host/pathogen prepared same reaction (monocytes internalised GBS) emphasis yielding sufficient pathogen RNA; which can sometime be impediment studies. Pathogen derived demonstrated amplify RT-qPCR 12 tested genes (cylE, 1010, rib, czcD, pil2B, cpsE, scpB, htp, cfb, copA, hvgA maeA). 4, used analyse differential gene expression mixed, RNA. Twelve assessed expression. Seven (IL8, IL1A, IL1B, IL10, TNF, LMO2 MCP-1) 6 (scpB, hvgA) significantly upregulated samples. Of tested, htp upregulated. An knockout mutant 874391 (Δhtp) constructed 5 assess impact transcription survival context. assays performed Δhtp construct. Contrary expectation, construct survived environment higher numbers than wild-type over 48 hours infection.