EFEITO ANTINATRIOREXIGÊNICO E ANSIOLÍTICO DA ADMINISTRAÇÃO INTRACEREBRAL DE CARVACROL EM RATOS: INFLUÊNCIA DAS VIAS SEROTONINÉRGICAS, ADRENÉRGICAS E POSSÍVEIS ÁREAS CEREBRAIS ENVOLVIDAS

摘要: The essential oil of oregano has among its main constituents carvacrol (70%). extract administered intraperitoneally in mice inhibits the reuptake monoamine neurotransmitters degradation (dopamine, serotonin and norepinephrine) a dose-dependent manner, increases levels several brain areas. orally showed significant increase all parameters observed anxiety test elevated plus maze. control water intake sodium appetite is done by complex interactive network stimulatory inhibitory circuits. It known that norepinephrine, as well serotonin, involved it unknown effects these behaviors. present study aimed to investigate effect intracerebroventricular administration on appetite, locomotor activity rats, FOS expression areas related Male Wistar weighing between 230-250g were anesthetized underwent stereotactic cannulation lateral ventricle (LV). day before experiments, rats received injections furosemide (20mg/kg, sc) low diet. animals CVC at concentrations 25, 50, 75, 150, 300 μg/5μl or vehicle hypertonic saline distilled measured for 1h. To involvement serotonergic adrenergic pathways, pCPA (200 μg/2μl) PRA (40, 80 160 nmol) VL microinjections CVC. deprived 12 hours was injected vehicle. A second group subjected maze 5 30 min after injection (150, 600 μg/5μl) room illuminated with red light intensity (3.2 lux). lasted minutes recorded video. following evaluated: number entries time spent arms apparatus. detect presence FOS, third transcardiac perfusion (0.01 M) paraformaldehyde (4%). brains removed processed identification FOS-IR nuclei counted "Image-Pro Discovery" software. data submitted mixed model two-way ANOVA Student's t test. Carvacrol inhibited way blockade alpha-adrenergic receptors attenuated inhibition induced central depletion also impairs antinatriorexigenic Regarding intake, did not change hours. μg resulted anxiolytic effect. Furthermore promotes increased areas: supraoptic nucleus, nucleus supra-chiasmatic, ventromedial hypothalamus, dorsomedial anterior periaqueductal gray lateral, dorsal ventrolateral regions, medial amygdala, basolateral, paraventricular thalamic accumbens. results this indicate anxiolytic effect, latter mediated noradrenergic pathways. They occur through neuronal activation various mainly hypothalamic who are behaviors studied