Regulation of expression of the human MTH1 gene encoding 8-oxo-dGTPase. Alternative splicing of transcription products.
作者： Hisanobu Oda , Yusaku Nakabeppu , Masato Furuichi , Mutsuo Sekiguchi
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摘要: The enzyme 8-oxo-7,8-dihydrodeoxyguanosine triphosphatase (8-oxo-dGTPase) hydrolyzes 8-oxo-dGTP to 8-oxo-dGMP, thereby preventing misincorporation of into DNA. We investigated expression MTH1 gene encoding 8-oxo-dGTPase. Large amounts mRNA were present in thymus and testis, embryonic tissues, certain cell lines. In peripheral blood lymphocytes, the level was significantly increased after concomitant treatment with phytohemagglutinin interleukin-2. Analyses 5' regions transcripts revealed that 7 types mRNAs, which may be produced by transcription initiation at different sites and/or alternative splicing. consists 5 major exons, some are composed differentially processed segments. All mRNAs carry entire coding region, functional. Three ATG codons in-frame found mRNAs. There is a polymorphic alteration splicing site (GT GC) located exon 2, an event affects patterns transcript. Allele frequency this polymorphism about 20% among healthy volunteers.
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sci-hub.se 参考文章(41)
YM Janssen, B Van Houten, PJ Borm, BT Mossman, Cell and tissue responses to oxidative damage. Laboratory Investigation. ,vol. 69, pp. 261- 274 ,(1993)
K C Cheng, D S Cahill, H Kasai, S Nishimura, L A Loeb, 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G----T and A----C substitutions. Journal of Biological Chemistry. ,vol. 267, pp. 166- 172 ,(1992) , 10.1016/S0021-9258(18)48474-8
T Bessho, K Tano, H Kasai, E Ohtsuka, S Nishimura, Evidence for two DNA repair enzymes for 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in human cells. Journal of Biological Chemistry. ,vol. 268, pp. 19416- 19421 ,(1993) , 10.1016/S0021-9258(19)36531-7
K Sakumi, M Furuichi, T Tsuzuki, T Kakuma, S Kawabata, H Maki, M Sekiguchi, Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis. Journal of Biological Chemistry. ,vol. 268, pp. 23524- 23530 ,(1993) , 10.1016/S0021-9258(19)49494-5
N. K. Bhat, C. B. Thompson, T. Lindsten, C. H. June, S. Fujiwara, S. Koizumi, R. J. Fisher, T. S. Papas, Reciprocal expression of human ETS1 and ETS2 genes during T-cell activation: regulatory role for the protooncogene ETS1. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 3723- 3727 ,(1990) , 10.1073/PNAS.87.10.3723
M. Moriya, Single-stranded shuttle phagemid for mutagenesis studies in mammalian cells: 8-oxoguanine in DNA induces targeted G.C-->T.A transversions in simian kidney cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 1122- 1126 ,(1993) , 10.1073/PNAS.90.3.1122
Tetsuya Kakuma, Jun-ichi Nishida, Teruhisa Tsuzuki, Mutsuo Sekiguchi, Mouse MTH1 protein with 8-oxo-7, 8-dihydro-2'-deoxyguanosine 5'-triphosphatase activity that prevents transversion mutation. cDNA cloning and tissue distribution Journal of Biological Chemistry. ,vol. 270, pp. 25942- 25948 ,(1995) , 10.1074/JBC.270.43.25942
M.H. Chung, H. Kasai, D.S. Jones, H. Inoue, H. Ishikawa, E. Ohtsuka, S. Nishimura, An endonuclease activity of Escherichia coli that specifically removes 8-hydroxyguanine residues from DNA. Mutation Research-dna Repair. ,vol. 254, pp. 1- 12 ,(1991) , 10.1016/0921-8777(91)90035-N
Kay Macleod, Dominique Leprince, Dominique Stehelin, The ets gene family Trends in Biochemical Sciences. ,vol. 17, pp. 251- 256 ,(1992) , 10.1016/0968-0004(92)90404-W
Financsek Istvan, Mizumoto Kiyohisa, Muramatsu Masami, Nucleotide sequence of the transcription initiation region of a rat ribosomal RNA gene. Gene. ,vol. 18, pp. 115- 122 ,(1982) , 10.1016/0378-1119(82)90109-3