Reduced RKIP expression levels are associated with frequent non-small cell lung cancer metastasis and STAT3 phosphorylation and activation.

摘要: The current study examined the role of Raf kinase inhibitor protein (RKIP) in non-small cell lung cancer (NSCLC) metastasis. A total 100 patients with NSCLC were recruited following pathological diagnosis First Affiliated Hospital Bengbu Medical College. classified and statistically analyzed according to their clinicopathological characteristics tumor-node-metastasis stage. Paired tumor tissue adjacent non-tumor samples subject western blot analysis. Transient transfection lentivirus particle vector-mediated RKIP overexpression, small interfering RNA-mediated silencing, Transwell assays immunocytochemistry methods employed elucidate underlying mechanisms Janus kinase/signal transducer activator transcription (JAK/STAT) signaling pathway Furthermore, order examine vivo effects RKIP, recombinant particles containing gene administrated a mouse model via tail vein injection. results revealed reduced expression levels compared corresponding non-cancer tissue. Additionally, inversely associated intra-lung, lymph node long-distance also indicated that was able block STAT3 activation phosphorylation inhibit NSCLC-cell metastasis vitro. knockdown promote lines. During experiments, overexpression suppress xenograft nude mice. Therefore, may be an important factor NSCLC, invasion by blocking JAK/STAT3 pathway.