Increased fluidity of Plasmodium berghei-infected mouse red blood cell membranes detected by electron spin resonance spectroscopy

摘要: Abstract The fluidity of Plasmodium berghei-infected mouse red cell membranes is increased over that uninfected cells at both 24°C and 37°C. This was demonstrated by electron spin resonance spectroscopy using the hydrocarbon labels 2-dodecyl-2′,5,5′-trimethyloxazolidine-N-oxyl 2-heptyl-2′ -hexyl-5,5′-dimethyloxazolidine-N-oxyl to label regions bilayer near its surface, deeper within region, respectively. Arrhenius plots ‘empirical motion parameter’ (Ri) obtained from 2-heptyl-2′-hexyl-5,5′-dimethyloxazolidine-N-oxyl-labeled versus temperature range 0 45°C showed an hysteretic behavior in mature immature cells. Such consistently lacking infected These differences membrane are interpreted reflect biochemical modifications which occur with infection malarial parasite.