Blue-green algal virus N-1: Physical properties and disassembly into structural parts

摘要: Abstract The structure of N-1, a virus infecting the filamentous, nitrogen-fixing blue-green alga Nostoc muscorem , has been further characterized. edge-to-edge distance N-1 head is 614 ± 28 A; length tail 1000 62 A. Flexible beaded fibers are attached to neck. Optical diffraction extended and contracted sheaths suggests that rearrangement protein subunits occurs upon contraction. molecular weight viral DNA calculated from sedimentation coefficient 44 3 × 10 6 . Based contour molecules, 41.8 3.6 A survey made effects number denaturing agents (urea guanidine hydrochloride, anionic cationic detergents, extremes pH, ultraviolet irradiation) native morphology. For each agent tested, first observable effect was trigger shortening (probably contraction) sheath. most resistant substructure From these investigations hierarchy increasing resistance chemical degradation could be arranged: capsid, core,