Viability of engineered biocatalysts in biotransformation

摘要: This project aims to exploit engineered biofilms as biocatalysts in the biotransformations of enantiomerically pure compounds for fine chemical and pharmaceutical industry. It conditions be designed which would improve reactions formation biofilms. Tsoligkas et al. (2012) has previously a biofilm act biocatalyst using tryptophan synthase, TrpBA produced from plasmid pSTB7 catalyse biotransformation haloindoles L-halotryptophans. To build on this work, how cells react were investigated through flow cytometry analysis colony forming units (CFU). For formed Escherichia coli (E. Coli) K-12, it was found that pT7-csgD had present or strain required an ompR234 point mutation allow production curli extracellular polymeric substances form biofilm. demonstrates importance CsgD regulator formation, without increase cellular concentration E. failed attach glass surfaces. From planktonic data is apparent carrying out with 5-chloroindole toxic effect metabolically active PHL644 pSTB7. The source toxicity not clear, may due products reaction, chloroindole being metabolised incorporated into proteins. Efflux indicates are incubated fluoroindole have decreased efflux, advantage biotransformation.