Phosvitin, a Phosphoglycoprotein I. ISOLATION AND CHARACTERIZATION OF A GLYCOPEPTIDE FROM PHOSVITIN
作者： Ruth Shainkin , Gertrude E. Perlmann
DOI: 10.1016/S0021-9258(19)77218-4
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摘要: Abstract The purified preparations of phosvitin, the phosphoprotein hens' egg yolk, contain 6.5% carbohydrate. Analysis showed 6 residues hexose, 5 glucosamine (assumed to occur as N-acetyl derivatives), and 2 sialic acid per molecular weight 40,000. In order determine mode attachment polysaccharide moiety, phosvitin has been digested with pronase. A sequence fractionation steps that included gel filtration, chromatography on DEAE-cellulose DEAE-Sephadex afforded isolation a glycopeptide containing all carbohydrate attached an aspartic residue. Edman degradation this peptide smaller isolated from tryptic digest partially dephosphorylated preparation yielded [see PDF for sequence] addition demonstration occurrence 8 phosphoserine residues, at least 3 nonphosphorylated serines were located near single moiety protein.
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