Effect of earthworms in reduction and fate of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs) during clinical laboratory wastewater treatment by vermifiltration.

摘要: Abstract In the recent decades, role of wastewater treatment plants has been entrenched for dissemination antibiotic resistant bacteria into environment. The present study explores dynamics earthworms-microorganisms interactions involved in high efficacy vermifiltration technology along with reduction (ARB). This is first its kind to investigate performance vermifilter (VF) clinical laboratory treatment. results showed that earthworms and VF associated microbial community had a significant effect on Biochemical Oxygen Demand (BOD) Chemical (COD) (78–85%), coliforms pathogen removal (>99.9%) caused shift prevalence pattern ARB. Molecular profiling resistance causing genes such as ESBL (blaSHV, blaTEM blaCTX-M), MRSA (mec-A) Colistin (mcr-1) confirmed probable mechanisms behind pattern. diversity influent, earthworm's coelomic fluid gut filter media layers assists formation biofilm, which helps pathogens from wastewater. biofilm further paradigm profile ARB ARG, specifically most effective against drugs, targeting cell wall protein synthesis inhibition Ampicillin, Ticarcillin, Gentamicin Chloramphenicol. These findings validate sustainable natural wastewater, resistance.