Next‐generation phylogenetics takes root
作者： John E. McCormack , Brant C. Faircloth
DOI: 10.1111/MEC.12050
关键词:
摘要: It has been a tumultuous 5 years in phylogeography and phylogenetics during which both fields have struggled to harness the power of next-generation sequencing (NGS) (Ekblom & Galindo 2010; McCormack et al. 2012a). Fortunately, several methodological approaches appear be taking root. In this issue Molecular Ecology, O'Neill 2013) employ one such method – parallel tagged (PTS) elucidate tiger salamander (Ambystoma tigrinum) species complex. This study demonstrates practical application NGS on scale appropriate (and not overkill) for most biologists interested (~100 loci ~100 individuals), their results highlight analytical challenges that lie ahead researchers employing techniques.
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