A novel hydrogen peroxide biosensor based on the specific binding of horseradish peroxidase with polymeric thiophene-3-boronic acid monolayer in hydrophilic room temperature ionic liquid
作者： Lin Cui , Minrong Xu , Jianying Zhu , Shiyun Ai
DOI: 10.1016/J.SYNTHMET.2011.06.004
关键词:
摘要: Abstract In the present work, we have developed a novel hydrogen peroxide (H 2 O ) biosensor based on electropolymerization of thiophene-3-boronic acid film (TBA) in ionic liquid via glucosidic bond with glycoprotein horseradish peroxidase (HRP). A thin poly (thiophene-3-boronic acid) (PTBA) has been synthesized by using room temperature liquids (RTIL) as growth medium and supporting electrolyte. Horseradish (HRP) this study shows affinity interaction formed PTBA film. With aid hydroquinone Q) mediator, fast response less than 7 s linear range 5.0 × 10 −6 to 1.75 × 10 −4 M detection limit 0.5 μM. The exhibits response, low limit, high stability very good reproducibility.
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