Interaction of DNA with connective tissue matrix proteins reveals preferential binding to type V collagen.

摘要: The interaction of DNA with type I to VI collagens and laminin was studied in vitro systems which the connective tissue components were immobilized, as well when solution. In studies on immobilized components, significant binding observed only for V collagen, radiolabeled this component could be effectively inhibited a concentration-dependent manner by addition unlabeled DNA. Similar results solution assays it that collagen dependent native triple-helical conformation collagen. preferential may due relative basicity chains, unique spatial arrangement amino acid side chains molecules. data are potential clinical relevance represent at least one mechanism whereby its immune complexes deposited tissues certain pathologic conditions.