Antimicrobial activity of Alcaligenes sp. HPC 1271 against multidrug resistant bacteria
作者： Atya Kapley , Himgouri Tanksale , Sneha Sagarkar , A. R. Prasad , Rathod Aravind Kumar
DOI: 10.1007/S10142-015-0466-8
关键词:
摘要: Alcaligenes sp. HPC 1271 demonstrated antibacterial activity against multidrug resistant bacteria, Enterobacter sp., to sulfamethoxazole, ampicillin, azithromycin, and tetracycline, as well Serratia GMX1, the same antibiotics with addition of netilmicin. The cell-free culture supernatant was analyzed for possible antibacterials by HPLC, active fraction further identified LC-MS. Results suggest production tunicamycin, a nucleoside antibiotic. draft genome this bacterial isolate analyzed, 4.2 Mb sequence data revealed six secondary metabolite-producing clusters, using antiSMASH platform ectoine, butyrolactone, phosphonate, terpene, polyketides, nonribosomal peptide synthase (NRPS). Additionally, homology tunicamycin-producing gene cluster also defined 30 ORFs linked protein secretion that could play role in observed. Gene expression analysis both NRPS dTDP-glucose 4,6-dehydratase clusters are functional be involved biosynthesis.
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