vir genes influence conjugal transfer of the Ti plasmid of Agrobacterium tumefaciens.

摘要: Mutation of the genes virA, virB, virC, and virG Agrobacterium tumefaciens octopine-type Ti plasmid pTiR10 was found to cause a 100- 10,000-fold decrease in frequency conjugal transfer this between cells. This effect not absolute, however, that it occurred only during early times (18 24 h) induction apparatus by octopine. Induction these mutant strains octopine for longer periods (48 72 resulted normal frequency. The vir gene mutations upon conjugation could be restored introduction cosmids harboring wild-type copies corresponding disrupted into strains. In addition, self-mobilizable pPH1JI impaired any tested. function on suggests relationship may exist processes control T-DNA from plant cells bacterial