Top-Down Atmospheric Ionization Mass Spectrometry Microscopy Combined With Proteogenomics.
作者： Cheng-Chih Hsu , Michael W. Baker , Terry Gaasterland , Michael J. Meehan , Eduardo R. Macagno
DOI: 10.1021/ACS.ANALCHEM.7B01096
关键词:
摘要: Mass spectrometry-based protein analysis has become an important methodology for proteogenomic mapping by providing evidence the existence of proteins predicted at genomic level. However, screening and identification directly on tissue samples, where histological information is preserved, remain challenging. Here we demonstrate that ambient ionization source, nanospray desorption electrospray (nanoDESI), interfaced with light microscopy allows profiling animal tissues microscopic scale. Peptide fragments mass spectrometry were obtained ganglia medicinal leech (Hirudo medicinalis) without in-gel digestion. We found a hypothetical protein, which genome, highly expressed specialized neural cells are uniquely in adult sex segmental ganglia. Via this top-down analysis, post-translational modification (PTM) tyrosine sulfation to neuropeptide was resolved...
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