Correlative assessment of fecal indicators using human mitochondrial DNA as a direct marker.

摘要: Identifying the source of surface water fecal contamination is paramount to mitigating pollution and risk human health. Fecal bacteria such as E. coli have been staple indicator organisms for over a century, however there remains uncertainty with coli-based metrics since these are abundant in environment. The relationships between presence direct waste (human mitochondrial DNA), human-specific Bacteroidales, were studied samples taken from an urban creek system (Duck Creek Watershed, Cincinnati, OH) impacted by combined sewer overflows. Logistic regression analysis shows that Bacteroidales correlates much more closely DNA (R = 0.62) relative 0.33). We also examine speciation within Duck Watershed using next-generation sequencing technology (Ion Torrent) show most numerous populations be associated sewage. Here we demonstrate human-spec...