Regional Control of Chromosome Segregation in Pseudomonas aeruginosa

摘要: Chromosome segregation in bacteria occurs concomitantly with DNA replication, and the duplicated regions containing replication origin oriC are generally first to separate migrate their final specific location inside cell. In numerous bacterial species, a three-component partition machinery called ParABS system is crucial for chromosome segregation. This case gammaproteobacterium Pseudomonas aeruginosa, where impairing very detrimental growth, as it increases generation time leads formation of anucleate cells mispositioning this study, we investigate vivo P. aeruginosa. Using chromatin immuno-precipitation coupled high throughput sequencing, show that ParB binds four parS site located within 15 kb vivo, binding promotes order nucleoprotein complex. We one enough prevent cell formation, therefore correct By displacing from its native position on chromosome, demonstrate chromosomal locus be separated upon which indicates force exertion process. identify region approximatively 650 surrounding must positioned proceed correctly, “competence zone” site. Mutant strains have undergone genetic rearrangements allow us propose distance between defines zone”. Implications control aeruginosa discussed.