Effects of low intensity pulsed ultrasound on rat Schwann cells metabolism.

摘要: The effects of low intensity pulsed ultrasound to tenocytes and osteocytes are well understood applied clinically. However, its on cultured Schwann cells still not elucidated. This study was designed elucidate the their possible molecular mechanism. were harvested from sciatic nerves 3-day-old Sprague-Dawley rats. Low stimulator (frequency: 1 MHz, duration: 2 min, duty cycle: 20%, total treatment time: 3 min) three different culture conditions: regular medium containing 0, 5, or 10% fetal bovine serum. viability, damage, differentiation examined; gene expression also analyzed. In presence 0.3 W/cm stimulation, increases in cell viability decreases apoptosis observed serum deprivation group; this condition, interleukin-1, tumor necrosis factor-alpha, protein zero genes downregulated Desert Hedgehog transcripts upregulated. We concluded that interven- tion with could promote proliferation, prevent death, keep adequate phenotype presentation for peripheral nerve recovery. stimulation an injured site be as early especially when microenvironment is almost serum-free obtain most benefit. Key Words: ultrasound—Schwann cells—gene expression—Nerve regeneration.