Yeast artificial chromosome cloning of a two-megabase-size contig within chromosomal band 18q21 establishes physical linkage between BCL2 and plasminogen activator inhibitor type-2.
作者： Gary A. Silverman , Jennifer I. Jockel , Peter H. Domer , Rose M. Mohr , Patricia Taillon-Miller
DOI: 10.1016/0888-7543(91)90245-A
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摘要: Abstract The construction of large-scale physical maps requires efficient approaches to generate new probes and link informative markers. mapping a human chromosomal segment was initiated by using the 18q21.3 probes, plasminogen activator inhibitor type-2 (PLANH2) BCL2, screen yeast artificial chromosome (YAC) library. An inverse polymerase chain reaction technique rescued genomic ends YAC inserts. These were used in walking strategy which established that PLANH2 gene 600 kb telomeric opposite transcriptional orientation BCL2. Overall, 16 YACs with mean size ∼300 analyzed rare-cutting restriction endonucleases 10 end-rescued probes. A contiguous map within spans approximately 2 Mb assembled. This establishes feasibility cloning surveying expanses genome lacking closely spaced, genetic landmarks.
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