Identification of adenosine A1 receptor ligands from Morus alba L. stem bark by NMR metabolomics approach

摘要: Comprehensive extraction coupled to NMR metabolomics was applied the identification of active compounds from Morus alba stem bark binding adenosine A1 receptor. Orthogonal projection least square (OPLS) analysis used estimate which significantly correlate with activity. Based on loading bi-plot and Y-related coefficient plot, unsaturated fatty acid signals strongly The characteristic alkaloids prenylated aromatic found be abundant in this plant do not positively Characteristic two isolated (betulinic morusin) also have negative coefficients indeed they are inactive when tested. Aromatic without prenyl or methoxy units proposed ones. To further identify possible compounds, comprehensive needs optimized, particularly selection a solvents combination gives high resolution metabolites over fractions.