Expression of prostaglandin F2α (PGF2α) receptor and its isoforms in the bovine corpus luteum during the estrous cycle and PGF2α-induced luteolysis

摘要: Prostaglandin F2α (PGF2α) induces luteolysis via a specific receptor, PTGFR. Although PTGFR mRNA expression in the bovine corpus luteum (CL) has been studied previously, changes protein and its localization are not fully understood during life span of CL. In addition to full-length PTGFR, several types isoforms, such as PTGFRα (type I) PTGFRζ II), were reported CL, suggesting isoform-specific luteal action. Full-length CL increased from early mid-luteal phase decreased luteolysis, whereas remained stable. was localized both endothelial cells expressed similarly Like mRNA, also phases, PTGFRζ, but PTGFRα, regressing During PGF2α-induced mRNAs PTGFR,α rapidly (from 5 or 15 min after PGF2α injection), only 12 h later. Silencing using small interfering RNA prevented PGF2α-stimulated cyclooxygenase-2 (PTGS2) induction. By contrast, could stimulate vascular growth factor A (VEGFA) even when knocked down, thus that may PTGS2 VEGFA is stimulated other isoforms. Collectively, continually estrous cycle, implying function throughout this period. Additionally, expresses different have effects acting