摘要: Publisher Summary This chapter discusses various methods for metal substitution. Metal substitution can be performed by three conceptually different approaches: (1) removal of the native a binding agent and/ or competition with hydrogen ions at low pH, followed insertion new metal, (2) direct displacement first second and (3) biosynthesis metalloprotein under enriched conditions choice. The two applied in either crystalline solution states. choice method physical state protein will depend on solubility, ease removal, stability apoprotein. methodology involved approaches both states enzyme illustrated detail carboxypeptidase A. A few exemplary references are given to where these have been used other metalloproteins.
sci-hub.se 参考文章(24)
Joseph E. Coleman, Bert L. Vallee, Metallocarboxypeptidases: stability constants and enzymatic characteristics. Journal of Biological Chemistry. ,vol. 236, pp. 2244- 2249 ,(1961) , 10.1016/S0021-9258(18)64065-7
Barton Holmquist, Bert L. Vallee, Metal Substitutions and Inhibition of Thermolysin: Spectra of the Cobalt Enzyme Journal of Biological Chemistry. ,vol. 249, pp. 4601- 4607 ,(1974) , 10.1016/S0021-9258(19)42460-5
Wolfgang MARET, Inger ANDERSON, Helmut DIETRICH, Helga SCHNEIDER-BERNLOHR, Roland EINARSSON, Michael ZEPPEZAUER, Site-Specific Substituted Cobalt(II) Horse Liver Alcohol Dehydrogenases FEBS Journal. ,vol. 98, pp. 501- 512 ,(1979) , 10.1111/J.1432-1033.1979.TB13211.X
David C. Speckhard, Felicia Y. H. Wu, Cheng-Wen Wu, Role of the intrinsic metal in RNA polymerase from Escherichia coli. In vivo substitution of tightly bound zinc with cobalt. Biochemistry. ,vol. 16, pp. 5228- 5234 ,(1977) , 10.1021/BI00643A011
Klaus Breddam, Thomas J. Bazzone, Barton Holmquist, Bert L. Vallee, Carboxypeptidase of Streptomyces griseus. Implications of its characteristics. Biochemistry. ,vol. 18, pp. 1563- 1570 ,(1979) , 10.1021/BI00575A028
David S. Auld, Barton Holmquist, Carboxypeptidase A. Differences in the mechanisms of ester and peptide hydrolysis Biochemistry. ,vol. 13, pp. 4355- 4361 ,(1974) , 10.1021/BI00718A018
Thomas L. Coombs, Yoshimori Omote, Bert L. Vallee, The Zinc-binding Groups of Carboxypeptidase A* Biochemistry. ,vol. 3, pp. 653- 662 ,(1964) , 10.1021/BI00893A010
David E. Drum, Bert L. Vallee, Differential chemical reactivities of zinc in horse liver alcohol dehydrogenase. Biochemistry. ,vol. 9, pp. 4078- 4086 ,(1970) , 10.1021/BI00823A008
David S. Auld, Bert L. Vallee, Kinetics of carboxypeptidase A. II. Inhibitors of the hydrolysis of oligopeptides. Biochemistry. ,vol. 9, pp. 602- 609 ,(1970) , 10.1021/BI00805A022
Robert T. Simpson, Bert L. Vallee, Two differentiable classes of metal atoms in alkaline phosphatase of Escherichia coli. Biochemistry. ,vol. 7, pp. 4343- 4350 ,(1968) , 10.1021/BI00852A029