Amino acid analysis by dinitrophenylation and reverse-phase high-pressure liquid chromatography
作者： Robert C. Morton , Gerhard E. Gerber
DOI: 10.1016/0003-2697(88)90111-X
关键词:
摘要: The determination of amino acids has been achieved by reverse-phase high-pressure liquid chromatography their dinitrophenyl derivatives. methods developed permit the quantitation all commonly encountered in a protein hydrolysate and effect various parameters on this separation was systematically evaluated. procedure eliminates need for specialized postcolumn equipment as employed conventional acid analysis can be obtained simple gradient chromatograph. sensitivity is comparable to that available common usage, being able determine quantitatively low picomole range.
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