Detection of Leptospira-Specific Antibodies Using a Recombinant Antigen-Based Enzyme-Linked Immunosorbent Assay

摘要: We produced three highly purified recombinant antigens rLipL32, rLipL41, and rLigA-Rep (leptospiral immunoglobulin-like A repeat region) for the detection of Leptospira-specific antibodies in an enzyme-linked immunosorbent assay (ELISA). The performance these was evaluated using 121 human sera. Among them, 63 sera were microscopic agglutination test (MAT)-confirmed positive from febrile patients Peru, 22 indigenous MAT-negative patient sera, 36 with other diseases Southeast Asia, where leptospirosis is also endemic. Combining results immunoglobulin M (IgM) IgG antigens, overall sensitivity close to 90% based on MAT. These suggest that ELISA multiple may be used as alternative method antibodies.