Purification and characterisation of two alpha-glucosidases from termite workers macrotermes bellicosus (termitidae: macrotermitinae)
作者： Lucien Patrice Kouamé , Assoi Yapi Désiré Patrice Yapi , Kouassi Hubert Konan , E. J. Parfait Kouadio , Siaka Binaté
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摘要: Objectives: The objective was to purify and characterise beta-glucosidases from termite Macrotermes bellicosus workers for use in glycobiotechnology elucidate the role played by these enzymes degradation of plant material. Methodology results: Two were purified a three-step procedure consisting ion-exchange, size-exclusion hydrophobic interaction chromatography techniques. Beta-Glc A B had molecular weights estimated at 204 216 kDa, respectively, SDS-PAGE; 209 230 gel filtration. Both exhibited same temperature pH optima pnitrophenyl-beta-D-glucopyranoside hydrolysis stable 37°C. showed high specificity beta-glucosyl residue preferred glucose-beta-(1-4) linkages beta-(1-3), beta-(12) beta-(1-6) linkages. inhibited sulfhydryl-binding reagents. Conclusions application findings: isolated this study appear be distinct other known terms substrate low K M value cellobiose. physiological two digestive tract could digestion di-and oligosaccharides derived celluloses. used as tool structural analysis D-glucose containing oligosaccharide chains glycoproteins , glycolipids cellulose.
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