Integrating transcriptomics, proteomics, glycomics and glycoproteomics to characterize paclitaxel resistance in breast cancer cells.

摘要: Chemoresistance is a major factor driving breast cancer (BC) relapse and the high rates of cancer-related deaths. Aberrant levels glycans are closely correlated with chemoresistance. The essential functions in chemoresistance not systematically studied. In this study, an integrated strategy combination transcriptomics, proteomics, glycomics glycoproteomics was applied to explore dysregulation glycogenes, glycan structures glycoproteins cells. paclitaxel (PTX) resistant MCF7 cells, 19 differentially expressed N-glycan-related proteins were identified, which MGAT4A most significantly down-regulated, consistent decrease expression at mRNA level PTX treated BC Glycomic analysis consistently revealed suppressed multi-antennary branching using MALDI-TOF/TOF-MS lectin microarray. Several target bearing ERK signaling pathway strongly Our findings demonstrated aberrant their on resistance. Systematically integrative multi-omic expected facilitate discovery glycosyltransferases, N-glycosylation tumor progression SIGNIFICANCE: An crucial understand association between BC. analysis, we identified unique glycan-related protein, glycoprotein signatures defining This study might provide valuable information molecular mechanisms underlying