Enrichment and Isolation

摘要: A section of this chapter focuses on the use enrichment and cultivation procedures with molecular methods, such as wholecell fluorescent in situ hybridization (FISH) or denaturing gradient gel electrophoresis (DGGE), to monitor progress an enrichment, evaluate presence contaminants, identify new isolates. Biophysical enrichments make conditions growth temperature, heat treatment, sonic oscillation, UV irradiation kill inhibit rest population. Biological may specific hosts for selective a particular organism, they take advantage some pathogenic property, invasiveness, which population does not possess. Bacteria are usually isolated from cultures by spatially separating organisms solid medium subsequently allowing them grow into colonies. The is designed demonstrate multiplicity many instances considerable ingenuity isolation methods bacteria presenting selected examples. buoyant density pure culture samples natural aquatic environments has been studied centrifugation Percoll gradients, average representative bacterium 1.080 pg µm -3. Isolation Legionella species, particularly those environment, can sometimes be facilitated acidification pH 2.2, kills contaminants more quickly than it species.