Optimization of lipase production from organic solid waste by anaerobic digestion and its application in biodiesel production

作者: P. Selvakumar , P. Sivashanmugam

DOI: 10.1016/J.FUPROC.2017.04.020

关键词:

摘要: Abstract Lipases are particularly important due to the fact that they specifically hydrolyze acylglycerols into free fatty acids and glycerol, which is of greater interest for biodiesel production. In this study, garbage lipase was produced from organic waste (Pomegranate (P), Orange (O) Pineapple (PA) peels) by optimization composition, ultrasonic pre-treatment time process parameters. Optimization parameters using RSM ANN modelling performed with composition 35(P): 20(O): 35(PA) 15 min (pre-treatment). The maximum activity 57.43 U/mL obtained at pH (6), temperature (33 °C), agitation (210 rpm) (4 days). effects pH, temperature, solvents on stability partially purified (PPL) enzyme kinetic were also investigated. PPL yielded 88.63% conversion palm oil biodiesel. Hence, would be a potential biocatalyst other industrial catalytic processes.

参考文章(41)
Lynn M. Hamilton, Catherine T. Kelly, William M. Fogarty, Purification and properties of the raw starch-degrading α- amylase of Bacillus sp. IMD 434 Biotechnology Letters. ,vol. 21, pp. 111- 115 ,(1999) , 10.1023/A:1005413816101
Moein Esmaeili, Mahmoud Yolmeh, Ahmad Shakerardakani, Hamid Golivari, A central composite design for the optimizing lipase and protease production from Bacillus subtilis PTCC 1720 Biocatalysis and agricultural biotechnology. ,vol. 4, pp. 349- 354 ,(2015) , 10.1016/J.BCAB.2015.05.002
Perinaz Bhada-Tata, Daniel A. Hoornweg, What a Waste : A Global Review of Solid Waste Management World Bank, Washington, DC. pp. 1- 116 ,(2012)
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Esra Uçkun Kiran, Antoine P. Trzcinski, Yu Liu, Glucoamylase production from food waste by solid state fermentation and its evaluation in the hydrolysis of domestic food waste Biofuel Research Journal. ,vol. 1, pp. 98- 105 ,(2014) , 10.18331/BRJ2015.1.3.7
Akhil Bajaj, Purva Lohan, Prabhat N. Jha, Rajesh Mehrotra, Biodiesel production through lipase catalyzed transesterification: An overview Journal of Molecular Catalysis B-enzymatic. ,vol. 62, pp. 9- 14 ,(2010) , 10.1016/J.MOLCATB.2009.09.018
Muhammad Tariq, Saqib Ali, Fiaz Ahmad, Mushtaq Ahmad, Muhammad Zafar, Nasir Khalid, Mir Ajab Khan, Identification, FT-IR, NMR (1H and 13C) and GC/MS studies of fatty acid methyl esters in biodiesel from rocket seed oil Fuel Processing Technology. ,vol. 92, pp. 336- 341 ,(2011) , 10.1016/J.FUPROC.2010.09.025
A. Robles-Medina, P.A. González-Moreno, L. Esteban-Cerdán, E. Molina-Grima, Biocatalysis: towards ever greener biodiesel production. Biotechnology Advances. ,vol. 27, pp. 398- 408 ,(2009) , 10.1016/J.BIOTECHADV.2008.10.008
Yuji Shimada, Chigusa Koga, Akio Sugihara, Toshihiro Nagao, Nobuo Takada, Susumu Tsunasawa, Yoshio Tominaga, Purification and characterization of a novel solvent-tolerant lipase from Fusarium heterosporum Journal of Fermentation and Bioengineering. ,vol. 75, pp. 349- 352 ,(1993) , 10.1016/0922-338X(93)90132-R