Microdialysis in Metabolic Research

摘要: Clinical metabolic research needs tools to get a footprint of human physiology and pathophysiology in vivo. The novel idea monitoring interstitial fluid by microdialysis the brain laboratory animals was complement ordinary blood sampling. Semipermeable hollow fibers were implemented brain-tissue mimicking characteristics artificial vessels communicating with freely diffusing molecules situ. Subcutaneous for measurements concentrations any low molecular compound introduced 1987. Each catheter required calibration situ “no net flux” method “true” glucose, lactate, adenosine, glycerol concentrations. Addition 133Xe-clearance assessments adipose tissue flow (ATBF) allowed adoption Fick’s principle an estimate substrate release. By time, technical improvements probes recovery techniques have made it possible monitor insulin cytokines/chemokines both sc muscle. In fact, almost every organ body may be accessible small large fluid. Thus, future technique looks bright.