SPOCK1 is a novel transforming growth factor-β target gene that regulates lung cancer cell epithelial-mesenchymal transition

摘要: Lung cancer is the leading cause of related death worldwide and prognosis still poor with 5-year survival approximately 15%. Metastasis by cancer. Recent researches have demonstrated that epithelial-to-mesenchymal transition (EMT) plays a key role in early process metastasis cells. Here, we identified SPARC/osteonectin, cwcv kazal-like domains proteoglycan 1 (SPOCK1) novel transforming growth factor-β1 (TGF-β) target gene regulates lung cell EMT. TGF-β has been reported as major inductor We observed expression SPOCK1 tumor tissues significantly higher than matched normal tissues. Moreover, was also non-metastasis Levels mRNA were increased among patients shorter disease-free times, indicating potential progression metastasis. Silencing endoribonuclease-prepared small interfering RNA (esiRNA) cells inhibits growth, colony formation invasion vitro. Interestingly, ectopic epithelial induced EMT mesenchymal marker Vimentin decreased E-cadherin. found under treatment TGF-β, downstream TGF-β. Taken together, our study showed biomarker may be new diagnostic therapeutic for