Novel electrochemical nuclear factorkappa B biosensor via analyte-restrained peptide nucleic acid displacement reaction constructed on different gold nanoparticle-deposited carbon electrodes
作者： Yuanyuan Xu , Ying Cai , Jinfeng Miao , Yazhi Yang , Weizhong Li
DOI: 10.1016/J.SNB.2021.129702
关键词:
摘要: Abstract In this paper an electrochemical biosensor for NF-κB based on analyte-restrained peptide nucleic acid (PNA) displacement reaction is proposed, which obviously more suitable DNA binding proteins’ detection compared with previous exonuclease cleavage methods. our system, PNA probe modified gold nanoparticles (AuNPs) deposited carbon electrodes could competitively conjugate one strand of the designed dsDNA forming a PNA/DNA duplex. Methylene blue (MB), as signal molecule, specifically inserts into duplex significantly amplifying signal. When present in it able to be recognized and bound against restraining formation duplex, thus responding decrease used analyze concentration NF-κB. Then, performances two different electrodes, glassy electrode (GCE) screen-printed (SPCE), are compared. The portable SPCE better analysis performance has low limit at 2.7 pg mL−1, determining S. uberis-infected MECs sample. Benefit from commercialized mass production, holds promising prospect practical application studying NF-κB-associated pathways diagnosing inflammatory related diseases.
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