Application of response surface methodology for rapid chrysene biodegradation by newly isolated marine-derived fungus Cochliobolus lunatus strain CHR4D.

作者: Jwalant K Bhatt , Chirag M Ghevariya , Dushyant R Dudhagara , Rahul K Rajpara , Bharti P Dave

DOI: 10.1007/S12275-014-4137-6

关键词:

摘要: For the first time, Cochliobolus lunatus strain CHR4D, a marine-derived ascomycete fungus isolated from historically contaminated crude oil polluted shoreline of Alang-Sosiya ship-breaking yard, at Bhavnagar coast, Gujarat has been reported showing rapid and enhanced biodegradation chrysene, four ringed high molecular weight (HMW) polycyclic aromatic hydrocarbon (PAH). Mineral Salt Broth (MSB) components such as ammonium tartrate glucose along with pH trace metal solution have successfully optimized by Response Surface Methodology (RSM) using central composite design (CCD). A validated, two-step optimization protocol yielded substantial 93.10% chrysene degradation on 4th day, against unoptimized 56.37% 14th day. The results depict 1.65 fold increase in 1.40 biomass considerable decrement time. Based successful laboratory experiments, C. CHR4D can thus be predicted potential candidate for mycoremediation HMW PAHs impacted environments.

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