Diversity of Karyolysus and Schellackia from the Iberian lizard Lacerta schreiberi with sequence data from engorged ticks.

摘要: Apicomplexan haemoparasites of the genera Schellackia Reichenow, 1919, and Karyolysus Labbe, 1894, seem to be common in lizards widespread across world. For decades, their identification has been based on morphological descriptions life cycle patterns, with molecular characterizations, applied only recently. We used characterization confirm detected by microscopy blood smears Lacerta schreiberi Bedriaga, 1878, a lizard Iberian Peninsula. Since samples other than were not available from studied lizards, 264 engorged ticks Ixodes ricinus (Linneaus, 1758) collected them as an alternative non-invasive source haemoparasite DNA for genetic analyses. Of 48 microscopically examined, 31 positive parasites (64.6% prevalence). identified trophozoites gamonts similar lacazei (Labbe, 1894) (24/48; 50%) Schellackia-like sporozoites (20/48; 41.7%). Mixed infections both species occurred 13 (27.1%). Sequence data obtained ticks. Phylogenetic analyses placed our unique haemogregarine sequence within clade, nevertheless, substantial polytomy. Thus, according its morphology effect host cell, we refer this cf. lacazei. Besides sequences being identical previously haplotype, also three new closely related haplotypes.