Activation of adenosine A3 receptors regulates vitamin C transport and redox balance in neurons.

摘要: Adenosine is an important neuromodulator in the CNS, regulating neuronal survival and synaptic transmission. The antioxidant ascorbate (the reduced form of vitamin C) concentrated CNS neurons through a sodium-dependent transporter named SVCT2 participates several processes, for instance, regulation glutamate receptors functioning synthesis neuromodulators. Here we studied interplay between adenosinergic system transport neurons. We found that selective activation A3, but not A1 or A2a, adenosine modulated transport, decreasing intracellular content. Forster resonance energy transfer (FRET) analyses showed A3 associate with SVCT2, suggesting tight signaling compartmentalization SVCT2. increased release SVCT2-dependent manner, which largely altered redox status without interfering cell death, glycolytic metabolism, bioenergetics. Overall, by C (via receptors) can regulate bioavailability control balance