Permeability of the suberized mestome sheath in winter rye.

摘要: Mestome sheath cells of winter rye (Secale cereale L. cv Puma) deposit suberized lamellae in their secondary cell walls. Histochemical tests including acid digestion and staining with Sudan IV Chelidonium majus root extract were used to detect the presence suberin primary wall. There was no evidence a Casparian band between adjacent mestome cells. Fluorescent dye techniques trace solute movement through leaf apoplast. Calcofluor white M2R, fluorescent which binds walls as it moves apoplastically, proved be too limited its mobility leaves test permeability. Trisodium 3-hydroxy-5,8,10 pyrene trisulfonate, is mobile apoplast, moved easily up vascular bundles transpiration stream, diffused outward from veins epidermal within minutes reaching particular level leaf. We conclude that freely permeable solutes moving apoplastically radial