作者: M.Luisa R�a , Antonio Ballesteros
DOI: 10.1007/BF00207628
关键词:
摘要: We describe a two-step method for the purification of two lipases (lipases A and B) from C. rugosa. The procedure includes Phenyl-Sepharose Sephacryl HR 100 chromatographies. enzymes obtained were pure according to criteria specific activity neutral sugar content.