Evidence from peptidomic analysis of skin secretions that allopatric populations of Xenopus gilli (Anura:Pipidae) constitute distinct lineages.

摘要: Abstract The International Union for Conservation of Nature (IUCN) Endangered Cape Platanna Xenopus gilli inhabits disjunct ranges at the tip Peninsula and near town Kleinmond on opposite sides False Bay in extreme southwest Africa. Peptidomic analysis host-defense peptides norepinephrine-stimulated skin secretions from frogs range resulted identification two magainins, peptide glycine–leucine–amide (PGLa) peptides, xenopsin-precursor fragment (XPF) nine caerulein-precursor (CPF) a related to glycine–glutamine (PGQ) previously found an extract laevis stomach. primary structures indicate close phylogenetic relationship between X. but only magainin-1, PGLa one CPF are identical both species. Consistent with previous data, show greatest antimicrobial potency hemolytic. There appreciable differences expression genes population animals sampled as corresponding magainin-G2, XPF-G1, XPF-G2, four present frogs, were not identified frogs. Conversely, PGLa-G3, XPF-G3, three animals. data support conclusion morphometric analyses comparisons nucleotide sequences mitochondrial that populations have undergone genetic, morphological, phenotypic divergence.