Effect of Environmental Conditions on Intracellular pH of Hybridoma Cells Cultured in Bioreactors
作者： M. Cherlet , P. Franck , J. M. Engasser , A. Marc
DOI: 10.1007/978-94-011-0437-1_22
关键词:
摘要: A procedure to measure the intracellular pH (pHi) of hybridoma cells cultivated inside batch and continuous bioreactors under controlled operating conditions was developed. Analysis done by flow cytometry using sensitive dye BCECFAM. Under physiological medium metabolite levels, a relationship found in various cultures between pHi cell specific growth rate. adverse conditions, when were exposed lower or higher ammonium ion concentration, conserved same value despite inhibition.
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sci-hub.st 参考文章(3)
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