The ras-like yeast YPT1 gene is itself essential for growth, sporulation, and starvation response.

摘要: The Saccharomyces cerevisiae gene YPT1 encodes a protein that exhibits significant homology to the mammalian ras proteins. Using disruption techniques, we have shown intact is required for spore viability. Lethality caused by loss of function, unlike yeast homologs RAS1 and RAS2 not suppressed bcy1 mutation, suggesting does act through adenylate cyclase regulatory system. A cold-sensitive allele, ypt1-1, was constructed. At nonpermissive temperature, mutants died, exhibiting aberrant nuclear morphology, as well abnormal distribution actin tubulin. mutant cells died without classical cell-cycle-specific arrest; nevertheless, examination cellular DNA content suggests function required, particularly after S phase. Cells carrying ypt1-1 mutation upon nitrogen starvation even at temperature permissive growth; diploid homozygous did sporulate. thus involved in nutritional regulation cell cycle normal progression mitotic cycle.