Evaluation of immunological markers in serum, filter-paper blood spots, and saliva for dengue diagnosis and epidemiological studies.
作者： Angel Balmaseda , Saira Saborio , Yolanda Tellez , Juan Carlos Mercado , Leonel Pérez
DOI: 10.1016/J.JCV.2008.07.016
关键词:
摘要: Abstract Background Numerous immunological approaches exist to diagnose dengue or detect virus (DENV) infections. Objectives To determine the best markers and specimen types for diagnosis measuring incidence of DENV infection in community-based studies. Study Design In one study, acute- convalescent-phase samples were collected from hospitalized suspected pediatric cases Managua, Nicaragua, September 2003 February 2004. A second study examined specimens a community setting Managua before after 2003–2004 season measure infection. both studies, detection anti-DENV IgM, IgA, IgG serum, filter-paper blood spots, saliva was compared gold standard performed on serum samples. Results For diagnosis, highest sensitivity specificity obtained by IgM IgA spots; intermediate poor results respectively. Detection alone functioned Conclusions spots yielded optimal cases, whereas marker
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