Identification of heat-stable A-factor from Myxococcus xanthus.

摘要: The asg mutants of Myxococcus xanthus fail to produce a set related substances called A-factor. A-factor is released into the medium and required early in fruiting body development. Lacking A-factor, are defective aggregation, sporulation, expression most genes whose products appear later than 1 h after development induced by starvation. Previous work has shown that these defects reversed when developing wild-type cells, added mutant cells. Part material conditioned with activity heat stable dialyzable. This low-molecular-weight consists mixture amino acids peptides. Fifteen single have activity, 11 found medium. Mixtures total approximately equal sum activities their constituents. Conditioned also contains peptides activity. Pure specific or less constituent acids. There no evidence for specialized peptide medium, one greater its About half heat-stable can be accounted free acids, remaining It argued induces A-dependent gene not nutritional action but through chemosensory circuit.