Enhanced mechanical properties of thermosensitive chitosan hydrogel by silk fibers for cartilage tissue engineering.

摘要: Abstract Articular cartilage has limited repair capability following traumatic injuries and current methods of treatment remain inefficient. Reconstructing provides a new way for natural polymers are often used as scaffold because their biocompatibility biofunctionality. In this study, we added degummed chopped silk fibers electrospun to the thermosensitive chitosan/glycerophosphate hydrogels reinforce two hydrogel constructs which were hyaline regeneration. The gelation temperature time analyzed by rheometer vial tilting method. Mechanical characterization was measured uniaxial compression, indentation dynamic mechanical analysis assay. Chondrocytes then harvested from knee joint New Zealand white rabbits cultured in constructs. cell proliferation, viability, production glycosaminoglycans collagen type II assessed. results showed that properties significantly enhanced when hybrid with layers made. GAG cell-seeded scaffolds indicate support chondrogenic phenotype chondrocytes significant increase fiber–hydrogel composite content two-layer Col II. It concluded these modified could be employed tissue engineering.