Fate of Antibiotics and Antibiotic-Resistant Fecal Bacteria in Water and Sediments from the Contamination Source to the Estuary: Impact and/or Resilience? Resilience to Contamination by Antibiotics

摘要: The aim of this study based on a multidisciplinary research program (FLASH) (FLASH (FLuxes Antibiotic and Antibiotic-resistant bacteria the corresponding genes in Surface Hydrosystems) project founded by CNRS EC2CO/GIP Seine-Aval), associating chemists, hydrologists, clinical environmental microbiologists, was to monitor impact antibiotic prescription human veterinary practices water sediment contamination Escherichia coli (antibiotic resistance, integrons) Enterococci (diversity, genes) along medical center–WWTP–river continuum. For purpose, multi-residue chemical methodology developed order detect low levels 34 antibiotics. In center, main prescribed (amoxicillin) weakly found effluents. Along continuum, antibiotics decreased from 160 μg.L−1 (cefotaxime) hospital effluents 1 ng.L−1 (ofloxacin) river. These concentrations were too exert selective pressure (mg.L−1) antibiotic-resistant bacteria. same samples, occurrence E. those harboring class integrons significantly (p-value < 0.001) Among Enterococcus populations, faecium mainly isolated (from 89 98 %). All isolates center multiple resistant, containing erm(B) mef(A) genes, belonged hospital-adapted clonal complex 17 (CC17). relative proportion CC17 favor other subpopulations, less resistant water, only persistent compounds (quinolones, macrolides, sulfonamides), but they did not correspond major resistances (penicillins, tetracyclines).