Antiangiogenesis efficacy of nitric oxide donors.

摘要: Angiogenesis is a complex process involving endothelial cell migration, proliferation, invasion, and tube formation. Inhibition of these processes might have implications in various angiogenesis-mediated disorders. Because nitric oxide (NO) known to play key role vascular diseases, the present study was undertaken determine NO using donor, S-nitroso N-acetyl penicillamine (SNAP) glutathione (SNAG). The antiangiogenic efficacy donors examined vivo vitro model systems. studies demonstrated ability SNAP inhibit cytokine fibroblast growth factor (FGF2)-stimulated formation serum-induced proliferation. inhibitory effect on proliferation by concentrations above millimolar range associated with significant shifts concentration metabolites. Furthermore, mouse Matrigel implant chick chorioallantoic membrane (CAM) models, maximal (85-95% inhibition) (FGF2)-induced neovascularization both models. SNAG resulted 85% inhibition FGF2-induced when given at 5 mg/kg/day infusion minipumps during 14 days 87% angiogenesis induced FGF2 CAM administered single dose 50 microg. Thus, be useful tool for human tumor growth, or neovascular, ocular, inflammatory diseases.