Triphenylstannyl((arylimino)methyl)benzoates with selective potency that induce G1 and G2/M cell cycle arrest and trigger apoptosis via ROS in human cervical cancer cells

摘要: Metal complexes with organelle specificity and potent but selective cytotoxicity are highly desirable. A novel series of triphenylstannyl 4-((arylimino)methyl)benzoates (2-8) were obtained by the reactions 4-formylbenzoate [Ph3Sn(L1)] 1 primary aromatic amines. Two representative compounds (10, 11) also synthesized reacting aqua-triphenylstannyl 2-formylbenzoate [Ph3Sn(L9)(H2O)] (9) aniline p-fluoroaniline, respectively. These characterized elemental analysis, IR 1H, 13C 119Sn NMR spectroscopy, as well single-crystal X-ray diffraction for 5, 7-11 three pro-ligands. The in vitro cytotoxic activities 1-11 assessed using MTT tetrazolium dye assay against HeLa (human cervical) MDA-MB-231 (breast) cancer cells, IC50 values revealing high activity. Compared to cisplatin, exhibited enhanced efficacy, indicating their potential anticancer agents. Among these, 5 demonstrated maximum inhibition negligible effect on normal human embryonic kidney (HEK) cells. combined results DCFH-DA Hoechst 33342/PI nuclear staining assays, along flow cytometry show that they possess a dual mode action: They induced apoptotic cell death, attributable tin-assisted generation reactive oxygen species. Cell cycle analyses indicated exhibit growth may cause turbulences G1 G2/M phases.