[27] Assays for cytochrome P-450 peroxygenase activity
作者： Minor J. Coon , Robert C. Blake , Ronald E. White , Gerald D. Nordblom
DOI: 10.1016/0076-6879(90)86119-G
关键词:
摘要: Publisher Summary This chapter discusses assays for cytochrome P- 450 peroxygenase activity. Toluene, which yields benzyl alcohol in the 450-catalyzed reaction, is a useful substrate mechanistic studies. To determine formation at fixed time intervals, gas chromatographic assay employed. Cytochrome versatile enzyme that catalyzes numerous types of chemical reactions with variety substrates, including hydroxylation xenobiotics, such as drugs, pesticides, dyes, organic solvents, anesthetics, and carcinogens, well naturally occurring lipids, steroids, fatty acids, prostaglandins. Molecular oxygen serves natural donor when electrons are supplied to by nicotinamide adenine dinucleotide phosphate (NADPH) via proteins serve electron carriers. In case liver microsomes or reconstituted system, NADPH-cytochrome- reductase carrier overall reaction equimolar amounts substrate, O 2 , NADPH consumed hydroxylated H O, NADP formed. can be replaced hydroperoxides related artificial donors. Following discovery ability 450-containing microsomal suspensions promote oxygenation an expense alkyl hydroperoxide, other oxidants, peroxy periodate, iodosobenzene, iodobenzene diacetate, N-oxides, found function similar capacity.
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