Timing readout in paper device for quantitative point-of-use hemin/G-quadruplex DNAzyme-based bioassays

摘要: This work describes a quantitative point-of-use hemin/G-quadruplex DNAzyme-based assay that integrates simple timing detection motif with low-cost, portable microfluidic paper-based analytical devices (μPADs). The readout is based on the selective DNAzyme-mediated wettability change of paper from hydrophilicity to hydrophobicity. Its utility well demonstrated sensitive, specific K+ ion as model analyte in artificial samples real human serum samples. new method only requires ubiquitous cheap timer (or cell phone function) provide results. It could offer opportunities for development more peroxidase-mimicking bioassays are simple, affordable, portable, and operable by minimally-trained users broad applications especially resource-poor settings.