作者: Abhiksha Desai , Jonathan Krynitsky , Thomas J. Pohida , Huaying Zhao , Peter Schuck
DOI: 10.1371/JOURNAL.PONE.0155201
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摘要: Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions macromolecules from the analysis their migration in centrifugal fields while free solution. A key mechanical element AUC centerpiece, component sample cell assembly that mounted between optical windows to allow imaging seal solution column against high vacuum exposed gravitational forces excess 300,000 g. For sedimentation velocity it needs be precisely sector-shaped unimpeded radial macromolecular migration. During history great variety centerpiece designs have been developed for different types experiments. Here, we report centerpieces can now readily fabricated by 3D printing at low cost, materials, with customized designs. The new exhibit sufficient stability withstand highest rotor speeds sufficiently precise equilibrium Sedimentation experiments bovine serum albumin as reference molecule printed standard double-sector design result boundaries virtually indistinguishable those commercial epoxy centerpieces, coefficients well within range published values. statistical error measurement slightly above obtained epoxy, but still below 1%. Facilitated modern open-source fabrication paradigms, believe accessories spawn improvements experimental design, efficiency resource allocation.